History pages
History
Logo Project Logo A high performance bioinformatics pipeline for large-scale
human genomic variation studies
Annotation Release 105 / Ensembl v75 / dbSNP 141 Home separator DB Info separator Blog separator About Us separator Help separator FAQ separator History
navbar navbar

Upload Parameters

upload parameters with
close resize
News
  • 2014.10.22
    Update NCBI database to annotation release 105.
  • 2014.10.22
    Update dbSNP database to Build 141.
  • 2014.04.03
    Add new species: Sheep, Genome assembly Oar_v3.1.

Result Preview Enlarge





Primer Z:
streamlined primer design for promoters, exons and human SNPs
Tsai, M.F., Lin, Y.J., Cheng, Y.C., Lee, K.H., Huang, C.C., Chen, Y.T. and Yao, Adam (2007) PrimerZ: streamlined primer design for promoters, exons and human SNPs, Nucleic Acids Research, doi:10.1093/nar/gkm383 Full Text
Species
Query By
Primer for

Gene Name or Ensembl ID Task ID
Upload a File

ex. APOE or ENSG00000130203
Mask SNPs
Mask SNPs on target sequence before primer design (Replace SNPs by "N")
Promoter Region

Upstream of gene: valid format is 0~1000000
Maximum Exon Length
Maximum Length
Exon Flanking Sequences Length
Flanking sequences Length
Product Size Ranges
Excluded Regions
Email Notification
Advanced Options
General Primer Picking Conditions
Min: Opt: Max:
Min: Opt: Max:
Min: Opt: Max:
Min: Opt: Max:
Other Per-Sequence Inputs
E.g. 20,400: only pick primers in the 400 base region starting at position 20.
Objective Function Penalty Weights for Primers
Lt: Gt: Lt: Gt:
Lt: Gt:
Objective Function Penalty Weights for Primer Pairs
Lt: Gt: Lt: Gt:
Hyb Oligo (Internal Oligo)
Hyb Oligo (Internal Oligo) Per-Sequence Inputs
Hyb Oligo (Internal Oligo) General Conditions
Min: Opt: Max:
Min: Opt: Max:
Min: Opt: Max:
Objective Function Penalty Weights for Hyb Oligos (Internal Oligos)
Lt: Gt: Lt: Gt:
Lt: Gt: